Key player twisted his knee and will be out for a “few weeks”, according to Tottenham manager.
Alli will now miss England’s World Cup qualifier against Scotland on Friday and the friendly against Spain four days later.
The 20-year-old was set to play in Sunday’s north London derby against Arsenal but the midfielder sustained the injury in training on Saturday and was left out of the squad.
“He’ll be out for a few weeks. We hope it’s not a big issue. It’s bad luck he was in the team, he’s an important player but that’s football,” Pochettino told Tottenham’s official Twitter account.
“(It was) bad luck yesterday in the training session, he twisted his knee. We need to assess him today and tomorrow.”
England’s interim manager Gareth Southgate, who started Alli against both Malta and Slovenia last month, will announce his latest squad on Sunday evening.
The news also comes as another blow to Spurs, who have been without a number of key players this season including Harry Kane, Toby Alderweireld, Mousa Dembele, Eric Dier and Hugo Lloris.
Kane and Dembele both returned to start against Arsenal at the Emirates Stadium.
Alli is likely to miss Tottenham’s crunch Champions League group game against Monaco later this month, as well as Premier League matches against West Ham and Chelsea.
